STAP細胞、小保方晴子の論文にコピペ盗用が見つかった！！

netgeek 2014年2月27日

再現実験が1件も成功しておらず、次から次へと疑惑が浮上する小保方晴子氏が過去に書き上げた論文に、ドイツの論文からの盗用が見つかった。

コピペを見つけたのは小保方晴子のSTAP細胞論文の疑惑というブログの運営者。「太字部分がそっくりそのまま、コピペされた箇所で、塩化カリウムを意味する「KCl」が、なぜか「KC1」という意味不明な言葉になっている」と指摘。PDFの元論文をコピペするとなぜかこのような文字化けがおこるので、小保方氏がコピペしたのは間違いない。

論文タイトル：　”Stimulus-triggered fate conversion of somatic cells into pluripotency”

Nature 505, 641–647 (30 January 2014)

Karyotype analysis

Karyotype analysis was performed by Multicolor FISH analysis (M-FISH). Subconfluent STAP stem cells were arrested in metaphase by colcemid (final concentration 0.270 µg ml−1) to the culture medium for 2.5 h at 37 °C in 5% CO2. Cells were washed with PBS, treated with trypsin and EDTA (EDTA), re-suspended into cell medium and centrifuged for 5 min at 1,200 r.p.m. To the cell pellet in 3 ml of PBS, 7 ml of a pre–warmed hypotonic 0.0375 M KC1 solution was added. Cells were incubated for 20 min at 37 °C. Cells were centrifuged for 5 min at 1,200 r.p.m. and the pellet was re-suspended in 3–5 ml of 0.0375 M KC1 solution. The cells were fixed with methanol/acetic acid (3:1; vol/vol) by gently pipetting. Fixation was performed four times before spreading the cells on glass slides.

特許出願時の文章にも同じコピペ文が使われている。

論文タイトル：Multicolor karyotype analyses of mouse embryonic stem cells.

In Vitro Cell Dev Biol Anim. 2005 Sep-Oct;41(8-9):278-83.Guo J1, Jauch A, Heidi HG, Schoell B, Erz D, Schrank M, Janssen JW.1Institute of Human Genetics, University of Heidelberg, D-69120 Heidelberg, Germany.Materials and MethodsChromosome preparationMetaphase spreads of the ES cells were performed as follows. Subconfluent ES cells were arrested in metaphase by adding colcemid (final concentration 0.270 μg/ml) to the culture medium for 2.5 h at 37° C in 5% CO₂. Cells were washed with PBS, treated with trypsin-ethylenediaminetetraacetic acid (EDTA), resuspended into cell medium and centrifuged for 5 min at 1200 rpm. To the cell pellet in 3 ml of PBS, 7 ml of a prewarmed hypotonic 0.0375 M KCl solution was added. Cells were incubated for 20 min at 37° C. Cells were centrifuged for 5 min at 1200 rpm and the pellet was resuspended in 3–5 ml of 0.0375 M KCl solution. The cells were fixed with methanol/acetic acid (3:1, vol:vol) by gently pipetting. Fixation was performed four times prior to spreading the cells on glass slides.